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Roberto Diso, born in Rome in 1932, attended an art school, after which he studied architecture, but dropped out in order to focus on his career as a comic artist, which began in 1954 on the pages of Il Victorioso magazine in collaboration with Sant D’Amico. Later on he produced mostly war stories for the British publisher Fleetway, such as Zip Nolan in Lion Comic, and provided artwork for Lancelot and Dan Panther for Éditions Aventures et Voyages, again in cooperation with D’Amico. With the Giolitti studios he worked for the German market, drawing adult comics like Grendizer (1965), episodes of Goldrake and mystery comics.

The collaboration that will make him famous and continue througout his life is the one with Sergio Bonelli Editore, which began in 1974. Sergio ordered two episodes from Diso  for the Rodeo edition, and then made him a part of the Mister No artist line-up . He became the main artist of the series and also illustrated all of its covers starting from number 116. He debuted with the episode Man from Guyana (Mister No no. 6/7). In addition to working for Bonelli, Diso also drew for L’Eternauta magazine, creating the comic Rodo (both script and artwork), whose main character originally appeared in 1985 in the Giungla! magazine. The same magazine originally published a short story by Alfred Castelli, illustrated by Diso, for the series Zone X Presents called L’occhio senza palpebre (Eye Without Eyelids). For Comic Art, Diso illustrated Rudy X by Rinaldo Traini, as well as several own stories.

In the new millennium, he continued working for Bonelli. In 2003, he did the artwork for an episode of Tex Willer Maxi edition. In 2006, after Mister No stopped running, Diso worked on episodes of other editions of Tex (one almanac and two maxis), and regular episodes of The Hidden Face and Shanghai Devil by Gianfranco Manfredi. He also worked on episodes of the Romanzi a fumetti edition, The Mohican and Bloody Path. He remains active to this day.